CellChek® D|D+

Frequently Asked Questions &


Frequently Asked Questions

KonanCare enables customers to consult, securely, by appointment, with Konan’s on-staff experts for interpretation of specular microscopic images, and to receive assistance on more advanced topics and methods associated with Konan’s products (CellChek XL, EvokeDx, CellChek D+ and EB-10, Chart2020, and the RAPDx pupillograph). With current product models, a secure, permission-based login session can be initiated by the customer allowing both the physician and the KonanCare team member to simultaneously view and discuss actual images using secure internet web services. KonanCare assistance is provided without charge for customers during the initial warranty period or during any period of Service Agreement coverage. A unique service, only from Konan Medical USA.

Flex Center MethodKonan Center/Flex Center methods are patented and are the only FDA 510(k) cleared methods for eye bank systems.  Accuracy established by independent reading centers for FDA clinical studies is +/-2.5%

Each CellChek D+ comes with a 20um grid calibration disk.  Enter 100um length using this disk and click a button. The computer will indicate a known magnification for 100um length.  It also enables the operator to verify if calibration was properly completed.

If the cells in the sample area are generally uniform in size and shape, 30-50 cells should be enough. However, if there is variation in cell size, at least 75-100 cells should be counted. Konan’s multi-sampling capability enables the operator to choose up to 4 different locations in the central cornea for adequate sampling. Analysis from each frame can be automatically averaged.

The operator is able to view the proper height of the stage by using the focusing knob and placing a slit of light reflection from the endothelium in the middle of a green box.

CellChek D+ has a built-in thermometer which measures medium temperature in real-time. For optimal results, the preservation medium should be 24 – 26 degrees C.

At 4º C, biochemical and physiological metabolic activities of endothelial cells slow down significantly. As temperature is increased to ~ room temperature, (>24º C), pumping mechanism is reactivated.  If cornea has low functional reserve, it may take longer for activation to occur.


CellChek D+
Viewing Field
1000 x 750 µm
480 X 600 µm
Analysis Area (each multi-field area)
400 X 300 µm X 4 areas (max 480,000 µm2)
200 X 280 µm
Chamber Compatibility
Numedis Life 4C®, Krolman®, B+L®, Stephens®
Life 4C®, Alcon®, Bausch+Lomb®, Krolman®
Vial Dimensions
up to 35 mm diameter
up to 35 mm diameter
Thermometer Range
0° to 45° C 0° to 45° C
Stage Translation Ranges
X, Y, and Z = 16 mm, Tilt = 15°
X and Y = 16 mm, Z= 20 mm, Tilt: 5°
LED: primary wavelength 525 nm
Dual CMOS: Finder and cornea
Dual CMOS: Finder and Cornea
Digital data feed to computer
On-board LCD: temperature and pachymetry
100-240 VAC, 50/60 Hz, 50 VA
100-240 VAC, 50/60 Hz, 50 VA
280 (W) X 215 (H) X 265 (D) mm
200 (W) X 255 (H) X 220 (D) mm
Data Interface
USB 2.0
USB 2.0
7.5 kg (without computer)
6.1 kg (without computer)
Operating Conditions
Ambient temp: 10° to 40° C
Relative humidity: 30% to 85%
Atmospheric pressure: 70 to 106 kPa
Ambient temp: 10° to 40° C
Relative humidity: 30% to 85%
Atmospheric pressure: 70 to 106 kPa
CE Mark, In Vitro USA
CE Mark, In Vitro USA